Promising Immobilization of Industrial-Class Phospholipase A1 to Attain High-Yield Phospholipids Hydrolysis and Repeated Use with Optimal Water Content in Water-in-Oil Microemulsion Phase

In this study, we maximized the reactivity of phospholipids hydrolysis with immobilized industrial-class phospholipase A1 (PLA1) at desired water content in water-in-oil (W/O) microemulsion phase. The optimal hydrophobic-hydrophilic condition reaction media a hydrophobic enzyme is critical to realize maximum yields activity A1. It was attributed enzymes disliking surroundings as special molecular structure for reactivity. Immobilization PLA1 successfully achieved aid carrier (Accurel MP100) combination treatment using glutaraldehyde. yield over 90% based on simple adsorption. kinetically investigated through effect glutaraldehyde and W/O initial rate increased linearly an increasing concentration then leveled off 6% concentration. rate, which predominantly driven by organic phase, changed according typical bell-shaped curve respect molar ratio phospholipid. behaved similar way different concentrations. After 10 cycles repeated use, well sustained 40% creation final product. Accumulated after times repetition sufficient industrial applications. Immobilized has demonstrated potential biocatalyst production phospholipid biochemicals.